The Single Best Strategy To Use For flow cytometer
The Single Best Strategy To Use For flow cytometer
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Knowledge-enriched characterization of cell populations. Morphological facts from visuals can add on the richness of flow cytometry data. For example, the determine demonstrates an in any other case conventional apoptosis assay utilizing Annexin V and PI, including mobile imaging to characterize cells in Each individual population to reveal morphologically unique characteristics. These insights could not have already been received from multiplex staining by itself.
As samples are obtained within the Attune CytPix Flow Cytometer, the high-pace brightfield digital camera captures and stores pictures of detected activities at a charge nearly 6,000 visuals for each next, dependant upon the flow charge and image dimensions. For larger overall flexibility, Attune Cytometric Software allows you to alter the image capture frequency as required and select a specific gate for picture seize, if expected.
(B) RAW 264.seven macrophages are sometimes annotated inaccurately with regard to particle depend due to their complexity and noncircular borders. The quantity of singlets readily available for analysis boosts as soon as the user trains and employs a more correct design.
Find out new insights into your experimental information by visualizing populations applying put together fluorescence and morphology knowledge. Our software package interface wizards allow downsampling, concatenation (merged files) and dimensionality equipment which have been constructed to the software program for an intuitive, streamlined data Examination encounter.
"An image isn't really very truly worth a thousand dots, but it seriously helps to see visuals. It is excellent to discover photographs that correlate with staining. One example is, AnnexinV-constructive cells which might be morphologically various than nutritious cells, or CD14+ monocytes which have been larger sized and much more textured than CD3+/CD4+/CD14- lymphocytes.
Also, it can be illuminating to view the quantity of doublets slip through standard singlet gates, and great to validate the position of unexpected double-optimistic occasions in my analysis."
Pair your Attune flow cytometer using an autosampler for increased workflow effectiveness, and include robotic integration for maximum throughput.
Our researchers will style panels to suit your needs as many times you need—totally free, no product or service purchase essential.
The Attune NxT cytometer may be the reputable workhorse instrument your lab can rely upon for dependable and trustworthy overall performance. The Attune CytPix design incorporates a high-speed brightfield digital camera and AI-driven picture analysis computer software, with modern upgrades which includes equipment Understanding equipment and an intuitive user interface, enabling Increased information precision, precision, and a lot quicker insights into sample biology.
End users can watch function pictures in serious-time to confirm sample good quality, optimize protocols, and file the morphology of each and every mobile populace discovered for Examination. Imaging seize also Rewards from acoustic focusing, which helps you to posture the cells in order that a sharp, centered image is acquired.
AI-pushed automated image analysis While using the Attune Cytometric Software package interprets party characteristics into distinctive morphology parameters that may be combined with normal fluorescence and scatter parameters to expose extremely reproducible population changes and unique subsets.
Software Notice: Automatic impression Assessment decreases consumer-to-consumer variability in flow cytometry gating approaches
Crafted-in dimensionality reduction tools improved resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to substantiate anticipated inhabitants resolution in lysed complete human blood. Consumer can perspective gatherings from Each and every cluster for Visible confirmation of cell phenotypes.
Flexible payment strategy from Agilent Financial Remedies enable you to have the crucial instrumentation you may need for immediate project, or to expand your lab.
At the guts of each Attune Flow Cytometer lies our groundbreaking acoustic concentrating know-how, which makes use of sound waves to meticulously align cells within the get more info flow cell. This modern solution surpasses traditional hydrodynamic techniques, leading to faster processing speeds, Outstanding info excellent, and enhanced sample throughput.
The Attune Xenith cytometer can be a high-parameter spectral analyzer, giving each spectral unmixing and traditional flow abilities for extensive and functional mobile Investigation.
Agilent Value Promise ensures you not less than a decade of instrument use with the date you purchase. Otherwise, We are going to credit history you Together with the residual worth of the program toward an upgraded design.
What appeared to be a homogeneous populace is definitely much more assorted—an Perception that needs to be thought of when interpreting benefits.